Comparision of the number of reads in NBF and PBM samples for the transcripts selected for qPCR.

ΔΔCt:2∧-((CT sample-CT housekeeping gene1) - (CT calibrator - CT housekeeping gene2)).CT sample: average CT of 3 qPCR repeats of PBM gene.CT housekeeping gene1: average CT of 3 qPCR repeats of ribosomal protein S7 PBM.CT housekeeping gene2: average CT of 3 qPCR repeats of ribosomal protein S7 NBF.CT calibrator: average CT of 3 qPCR repeats of NBF gene.*significant, R>9. Trypsin, Arginase and Serine-Pyruvate Aminotransferase also significant by DESeq, p

ΔΔCt计算公式为：2^(-[(CT值（Cycle threshold，CT）样本 - CT值管家基因1) - (CT值校准物 - CT值管家基因2)])。各参数定义如下：CT值样本为PBM基因3次qPCR重复实验的CT值平均值；CT值管家基因1为核糖体蛋白S7 PBM的3次qPCR重复实验的CT值平均值；CT值管家基因2为核糖体蛋白S7 NBF的3次qPCR重复实验的CT值平均值；CT值校准物为NBF基因3次qPCR重复实验的CT值平均值。* 表示具有统计学显著性，且R>9。经DESeq分析，胰蛋白酶（Trypsin）、精氨酸酶（Arginase）及丝氨酸-丙酮酸氨基转移酶（Serine-Pyruvate Aminotransferase）同样呈显著差异，p