Dataset supporting Figure 2 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from the same dorsal mount mouse, corresponding to panels a-c and d of Figure 2, respectively. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data.
Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".
Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".
Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).
In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv).
Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".
Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.
Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.
Please contact the authors for any additional clarifications as needed.
See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513

本数据集包含来自同一只背侧植入式小鼠（dorsal mount mouse）的单次单光子宽场成像（1-photon widefield imaging）实验数据，以及单次Thorlabs双光子介观成像（mesoscope 2-photon imaging）会话数据，分别对应图2的a-c面板与d面板。收录文件涵盖成像数据、行为学数据，以及包含整合神经行为学数据的Python脚本文件。

请注意，会话名称遵循以下格式："mouse#_bigEndianDate_cage#_info_session#_attempt#"。

原始介观成像数据以ScanImage渲染格式存储，为单张大尺寸TIFF文件，命名规则为："filename_2D.tiff"。

小鼠面部与躯体摄像头影像以独立或拼接的.avi视频文件形式收录，行为学数据同时包含Spike2格式文件（后缀.smrx）与导出的Matlab数据文件（后缀.mat）。

所有数据均以LabVIEW发出的首个帧时钟触发信号为基准对齐，包括双光子影像的首帧、右侧面部/躯体影像的首帧，以及Spike2数据（该触发信号与所有其他帧时钟事件均已在Spike2中记录）。双光子触发信号有时会在Spike2中出现记录错误（通常我们会同时记录为事件与波形），但所有会话的该信号均可通过ScanImage的TIFF元数据导出为时间戳文件（以header.csv为后缀的CSV文件）。

会话起始时间戳同样从ScanImage的TIFF元数据中导出，存储为以"_starttime.txt"为后缀的TXT文件。

预处理数据（Python格式）存储于各类命名的.npy文件中，每个文件包含分析所需的不同变量子集。针对每个会话，文件名包含"standard_frames"字符串的.npy文件包含最完整的最终阶段预处理神经行为学数据（采用整合数据框（DataFrame）格式，可导出为NWB（Neurodata Without Borders）格式），其中包含CCF/MMM配准信息。文件名包含"nb_dump"字符串的文件则包含大量辅助变量，可用于后续额外预处理步骤。

本数据集同时收录额外的图像文件（TIFF、PNG格式）与Excel表格文件（XLSX、CSV格式），用于提供高层次数据汇总及中间分析步骤记录。

如有任何额外说明需求，请联系论文作者。

相关数据集材料可访问以下集合链接：https://doi.org/10.25452/figshare.plus.c.7052513