Gene expression changes associated with KIAA1199 expression in SW480 cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39575
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The aim was to identify gene expression changes upon expression of KIAA1199 in a colon cancer cell line. Inducible expression of KIAA1199A was achieved with a two-step procedure. First, SW480 cells were transfected with the pcDNA6TR vector (Invitrogen) and the tetracycline repressor-expressing cells selected with 7 μg/ml blasticidin S (InvivoGen). The blasticidin-resistant cells were then transfected with the pT-Rex-DEST30-KIAA1199 construct (see below) and selected with 0.4 mg/ml G418. The growth medium used for these cells was supplemented with 10% Tet-system-approved fetal calf serum (Biochrom).
本研究旨在明确结肠癌细胞系中KIAA1199表达后发生的基因表达变化。KIAA1199A的诱导表达通过两步法完成：第一步，将SW480细胞转染pcDNA6TR载体（Invitrogen），并以7 μg/ml杀稻瘟菌素S（InvivoGen）筛选获得稳定表达四环素阻遏蛋白的细胞；随后，将上述杀稻瘟菌素抗性细胞转染pT-Rex-DEST30-KIAA1199构建体（详见下文），并以0.4 mg/ml G418进行筛选。该类细胞使用的生长培养基添加了10%经Tet系统认证的胎牛血清（Biochrom）
创建时间：
2013-09-02
