Confocal Image Data Repository for the Study: “In Vivo High-Resolution MSSR Microscopy Uncovers the Intoxication Pathway of Bacillus thuringiensis Cry Toxins in the Insect Gut, Implicating Actin Depolymerization, Massive Endocytosis, and Vesicle Secretion”

This repository contains high-resolution confocal microscopy datasets of mosquito larval midgut tissues exposed to Bacillus thuringiensis Cry toxins and fluorescent markers for cellular organelles. The data were collected using an Olympus FV1000 confocal laser scanning microscope with a 60X oil immersion objective and a pixel size of 59 nm. The datasets include images of Cry toxins labeled with Alexa Fluor dyes and markers such as SiR-Actin, SiR-Lysosomes, MitoTracker, FM4-64, ER-Tracker, and DAPI. Each dataset represents independent replicates from midgut tissue dissections of at least five larvae per experimental condition.

本数据集仓库收录了经苏云金芽孢杆菌（Bacillus thuringiensis）Cry毒素处理的蚊幼虫中肠组织，以及用于标记细胞细胞器的荧光标记物的高分辨率共聚焦显微镜成像数据集。本数据集采用奥林巴斯（Olympus）FV1000共聚焦激光扫描显微镜采集，搭配60倍油浸物镜，像素尺寸为59 nm。数据集包含经亚历克斯荧光（Alexa Fluor）染料标记的Cry毒素图像，以及SiR-Actin、SiR-Lysosomes、MitoTracker、FM4-64、ER-Tracker与DAPI等荧光标记物的相关成像数据。每个数据集均对应各实验条件下至少5只幼虫的中肠组织解剖独立重复样本。