Hippocampal neuronal responses to programmable antigen-gated G-protein-coupled engineered receptor activation

This dataset contains single cell electrophysiological recordings of mouse hippocampal CA1 neurons in response to activation of programmable antigen-gated G-protein-coupled engineered receptors. Recorded neurons were transfected with an AAV1/2-hSyn-a-mCherry-PAGER-Gi-P2A-mEGFP and responses were then recorded in response to DCZ (100 nM) or DCZ + soluble mCherry (1 uM) application.

The authors are grateful to the St Jude Children’s Research Hospital Collaborative Research Consortium on GPCRs, the Chan Zuckerberg Biohub–San Francisco, Phil and Penny Knight Initiative for Brain Resilience (KIG-104), Stanford Cancer Institute, Wu Tsai Neurosciences Institute of Stanford University and the NIH (MH135934 to A.Y.T. and I.S., F32CA257159 to N.A.K., NS121106 to I.S.) for funding this work. R.T. was supported by the Life Sciences Research Foundation Fellowship (sponsored by Astellas Pharma) and JSPS Overseas Research Fellowship.

本数据集包含小鼠海马CA1神经元在可编程抗原门控G蛋白偶联工程受体激活下的单细胞电生理记录。所记录的神经元已转染AAV1/2-hSyn-a-mCherry-PAGER-Gi-P2A-mEGFP载体，随后通过施加DCZ（100 nM）或DCZ+可溶性mCherry（1 μM）记录其电活动响应。

本研究感谢圣犹大儿童研究医院G蛋白偶联受体（G protein-coupled receptor, GPCR）协作研究联盟、陈-扎克伯格旧金山生物中心（Chan Zuckerberg Biohub–San Francisco）、菲尔与彭妮·奈特脑康复倡议（KIG-104）、斯坦福大学癌症研究所、斯坦福大学吴蔡神经科学研究所，以及美国国立卫生研究院（National Institutes of Health, NIH）提供的研究经费支持，具体资助项目包括：授予A.Y.T.与I.S.的MH135934、授予N.A.K.的F32CA257159，以及授予I.S.的NS121106。R.T. 获得了生命科学研究基金会奖学金（由安斯泰来制药赞助）以及日本学术振兴会（Japan Society for the Promotion of Science, JSPS）海外研究奖学金支持。