Structure of human TRPM8 channel

This repository includes the following source datasets: 1. modeling.zip Modeling of icilin binding to HsTRPM8 (Figure 3e, 3f, 3g, 3h) - HsTRPM8-KX7_opt.pdb: original pose (Figure 3e, 3f) - HsTRPM8-KX7_opt_rotated.pdb: rotates pose (Figure 3g, 3h) 2. activation_assay.zip HsTRPM8 activation assay results (Figure 3i and Supplementary Figure 5) - HsTRPM8_activation_assay_results.pzf: GraphPad file with activation Assay results 3. Fr-TM.zip Structure-based multiple sequence alignment of TRPM channels (Supplementary Figure 4a) - Fr-TM_info.txt: description of procedure used to create alignment - TRPM_new_raw_pairwise_alignments.fas: raw pairwise alignment of new TRPM sequences to 6CO7 reference - TRPM_S6_restriction_site_aligned.fas: final multiple sequence alignment used to generate Supplementary Figure 4a 4. 3Dvar.zip HsTRPM8 3D Variability Analysis results (Supplementary Movie 1) - *.mrc files: composite maps with extreme frames for two analyzed components (aligned with focused MHR1/2 map) - *.pdb files: HsTRPM8 models fitted (MDFF) to above maps and used to generate Supplementary Movie 1 5. Supplementary_Movie_1.mp4 Morphing between different conformations of HsTRPM8 resolved by 3D Variability analysis (Supplementary Movie 1).本仓库包含以下源数据集： 1. modeling.zip 冰精素（icilin）与HsTRPM8的结合建模（对应图3e、3f、3g、3h） - HsTRPM8-KX7_opt.pdb：初始构象（对应图3e、3f） - HsTRPM8-KX7_opt_rotated.pdb：旋转后构象（对应图3g、3h） 2. activation_assay.zip HsTRPM8激活实验结果（对应图3i与补充图5） - HsTRPM8_activation_assay_results.pzf：包含激活实验结果的GraphPad格式文件 3. Fr-TM.zip TRPM通道的基于结构的多序列比对（对应补充图4a） - Fr-TM_info.txt：该多序列比对的构建流程说明文档 - TRPM_new_raw_pairwise_alignments.fas：新TRPM序列与6CO7参考序列的原始两两比对结果 - TRPM_S6_restriction_site_aligned.fas：用于生成补充图4a的最终多序列比对文件 4. 3Dvar.zip HsTRPM8三维变异性分析（3D Variability Analysis）结果（对应补充视频1） - *.mrc文件：包含两个分析组分极端帧的复合图谱（与聚焦型MHR1/2图谱对齐） - *.pdb文件：采用MDFF方法与上述图谱拟合，并用于生成补充视频1的HsTRPM8模型文件 5. Supplementary_Movie_1.mp4 通过三维变异性分析解析得到的HsTRPM8不同构象间的形态过渡动画（对应补充视频1）。