Comparative analysis of the cistromes of ERα and phosphorylated CREB1 in breast cancer cells

We report the genome-wide chromatin binding profiles of ERα and phosphorylated CREB1 (pCREB1) upon stimulation of human MDA-MB-134 breast cancer cells with estradiol or cAMP. We highlight the unique and the shared cistromes for each transcription factor. ChIP-seq of ERα and phosphorylated CREB1 with human MDA-MB-134 breast cancer cells; duplicate samples were treated with vehicle (DMSO), estradiol or cAMP

本研究报道了以雌二醇（estradiol）或环磷酸腺苷（cAMP）刺激人MDA-MB-134乳腺癌细胞后，雌激素受体α（ERα）与磷酸化CREB1（phosphorylated CREB1，pCREB1）的全基因组染色质结合谱。本研究阐明了两类转录因子各自独特的顺式调控元件组（cistrome）以及二者共有的顺式调控元件组。本研究针对人MDA-MB-134乳腺癌细胞中的ERα与磷酸化CREB1开展染色质免疫共沉淀测序（ChIP-seq）实验；实验设置生物学重复样本，分别以溶媒对照（二甲基亚砜，DMSO）、雌二醇或环磷酸腺苷进行处理。