Diurnal oscillations in murine brown adipose tissue analyzed by RNA-seq. Diurnal oscillations in murine brown adipose tissue analyzed by RNA-seq

Brown adipose tissue (BAT) burns fatty acids to produce heat, and shows diurnal oscillation with peak activity in glucose and triglyceride-derived fatty acid uptake at wakening. To gain further insight in the diurnal regulation of metabolic BAT activity, we performed RNA-sequencing (RNA-seq) on interscapular (i)BAT samples collected from chow-fed male C57BL/6J mice (10 weeks old) exposed to mild cold (22°C) at 3-hour intervals throughout a 24-hour period (n=4 mice per time point). All mice were entrained to a 12h:12h light:dark cycle, and therefore time is denoted as Zeitgeber Time (ZT) in which ZT0 indicates the onset of the light (active) phase. Overall design: We applied RNA-seq on total RNA isolated from murine iBAT obtained at 3 hour-intervals throughout a 24-hour period (8 time points in total; 4 replicates per time point).

棕色脂肪组织（Brown Adipose Tissue, BAT）可通过燃烧脂肪酸产热，其活性呈现昼夜节律振荡，在觉醒时刻的葡萄糖及甘油三酯衍生脂肪酸摄取过程中达到活性峰值。为深入探究棕色脂肪组织代谢活性的昼夜调控机制，我们对24小时内每3小时采集一次的肩胛间（interscapular, i）BAT样本进行了RNA测序（RNA-sequencing, RNA-seq）。样本取自以普通饲料喂养的10周龄雄性C57BL/6J小鼠，实验环境设置为轻度低温（22℃），每个时间点包含4只小鼠作为生物学重复（n=4）。所有小鼠均经12小时光照:12小时黑暗的光暗周期驯化，因此实验时间以授时因子时间（Zeitgeber Time, ZT）标注，其中ZT0代表光照（活动）相的起始时刻。实验整体设计：我们对24小时内每3小时采集的小鼠肩胛间BAT总RNA进行了RNA-seq测序，共涵盖8个时间点，每个时间点设置4次生物学重复。