Tatyana M. Svitkina, Alexander B. Verkhovsky,, Gary G. Borisy (2011) CIL:23041, Rattus, fibroblast. CIL. Dataset

Association of plectin with myosin II. A gelsolin-treated REF-52 cytoskeleton immunogold labeled myosin II (10 nm). After actin depletion, intermediate filaments with plectin sidearms remain associated with individual myosin bipolar filaments. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 10d from J Cell Biol. 1996 Nov;135(4):991-1007.

肌动蛋白结合蛋白与肌球蛋白II的关联研究。采用凝胶素处理的REF-52细胞骨架进行免疫金标记肌球蛋白II（10纳米）。在肌动蛋白耗竭后，带有肌动蛋白结合蛋白侧臂的中间纤维仍与单个肌球蛋白双极纤维保持关联。细胞骨架的电子显微镜观察依照Svitkina等（1995年）所述方法进行。简而言之，盖玻片上的细胞以光显微镜方法进行裂解，并使用重组凝胶素N-端结构域进行处理，随后用戊二醛、鞣酸和铀酸醋酸固定，临界点干燥，并镀铂和碳。图像对应于《细胞生物学杂志》1996年11月第135卷第4期的图10d。