gene profiling of turnip crinkle virus (tcv) sirna-Gene profiling of Turnip Crinkle Virus (TCV) siRNA in A.thaliana. Arabidopsis thaliana

rs10-05_tcv - gene profiling of turnip crinkle virus (tcv) sirna - 1. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in A.thaliana? 2. There are also differentially regulated during an evolution and a fitness process? - This is a plant evolution project on TCV in which, the biological questions are: 1. What are the genes (including miRNA precursors) that are differentially regulated in Col0 and dcl234 mutant in wt conditions? 2. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in Col0 and dcl234 mutant? 3. There are also differentially regulated between the plant generations 1 (G1) and 11 (G11)? 4. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA after fitness experiment? Overall design: 24 dye-swap - gene knock out,treated vs untreated comparison

rs10-05_tcv数据集——芜菁皱缩病毒(turnip crinkle virus, TCV)小干扰RNA(small interfering RNA, siRNA)基因表达谱分析。该数据集初始提出两项核心研究问题：1. 拟南芥(Arabidopsis thaliana, A.thaliana)中，受病毒小干扰RNA调控存在差异表达的基因（含miRNA前体(miRNA precursors)）有哪些？2. 同时在进化与适应度过程中存在差异调控的基因有哪些？本项目为针对TCV的植物进化研究，其细化的生物学问题如下：1. 野生型条件下，哥伦比亚野生型(Col0)与dcl234突变体植株中，存在差异表达的基因（含miRNA前体）有哪些？2. 哥伦比亚野生型(Col0)与dcl234突变体植株中，受病毒小干扰RNA调控存在差异表达的基因（含miRNA前体）有哪些？3. 植物第1代(G1)与第11代(G11)之间存在差异调控的基因有哪些？4. 经过适应度实验后，受病毒小干扰RNA调控存在差异表达的基因（含miRNA前体）有哪些？实验设计：包含24组染料互换(dye-swap)实验，用于对比基因敲除(gene knock out)处理组与未处理组的表达差异。