Table_2_Source Attribution Study of Sporadic Salmonella Derby Cases in France.xlsx

Salmonella enterica subsp. enterica serovar Derby is one of the most frequent causes of gastroenteritis in humans. In Europe, this pathogen is one of the top five most commonly reported serovars in human cases. In France, S. Derby has been among the ten most frequently isolated serovars in humans since the year 2000. The main animal hosts of this serovar are pigs and poultry, and white meat is the main source of human contamination. We have previously shown that this serovar is polyphyletic and that three distinct genetic lineages of S. Derby cohabit in France. Two of them are associated with pork and one with poultry. In this study, we conducted a source attribution study based on single nucleotide polymorphism analysis of a large collection of 440 S. Derby human and non-human isolates collected in 2014–2015, to determine the contribution of each lineage to human contamination. In France, the two lineages associated with pork strains, and corresponding to the multilocus sequence typing (MLST) profiles ST39-ST40 and ST682 were responsible for 94% of human contaminations. Interestingly, the ST40 profile is responsible for the majority of human cases (71%). An analysis of epidemiologic data and the structure of the pork sector in France allowed us to explain the spread and the sporadic pattern of human cases that occurred in the studied period.

肠炎沙门氏菌肠炎亚种德比血清型（Salmonella enterica subsp. enterica serovar Derby）是引发人类胃肠炎的最常见致病菌之一。在欧洲，该病原菌是人类感染病例报告数量排名前五的常见血清型之一。自2000年起，法国境内的人类感染病例中，德比沙门氏菌（S. Derby）始终位列分离频次最高的前十种血清型之列。该血清型的主要动物宿主为猪与家禽，白肉则是人类感染的主要污染源。我们此前的研究证实，该血清型为多系演化类群，法国境内共存有3个独立的遗传谱系，其中两个与猪源菌株相关，另一个与家禽源菌株相关。本研究针对2014至2015年采集的440株德比沙门氏菌人类及非人类分离菌株，开展了基于单核苷酸多态性（single nucleotide polymorphism, SNP）分析的传染源归因研究，以明确各遗传谱系对人类感染的贡献占比。结果显示，在法国，对应多位点序列分型（multilocus sequence typing, MLST）型别ST39-ST40与ST682的两个猪源谱系，造成了94%的人类感染病例。值得关注的是，ST40型别导致了其中71%的人类感染病例，为占比最高的致病谱系。通过对流行病学数据及法国猪肉产业结构的分析，我们得以阐明本研究时段内人类感染病例的传播态势与散发流行模式。