Parameters used for modeled curves shown in Figure 2.
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V: Velocity of transcription in nucleotides (nt) incorporated sec−1.P: Probability that pre-rRNA will undergo nascent transcript cleavage (NTC) rather than released transcript cleavage (RTC).NTC window: The distance traveled (in nt) by the transcribing polymerase downstream of site A2 prior to the NTC event.Equilibration time: Time required for [3H] tritium uptake and equilibration of the internal nucleotide pool, prior to linear incorporation of label into newly synthesized RNA.Lifetimes are in seconds.
V:转录速率,单位为每秒掺入的核苷酸(nucleotides, nt)数。 P:预核糖体RNA(pre-rRNA)发生新生转录本切割(nascent transcript cleavage, NTC)而非释放后转录本切割(released transcript cleavage, RTC)的概率。 NTC窗口:转录聚合酶在发生NTC事件前,于A2位点下游移动的核苷酸距离。 平衡时间:在标记物线性掺入新合成RNA之前,[3H]氚(tritium)摄取以及内部核苷酸池达到平衡所需的时间。 寿命单位为秒。




