RNA-seq in livers of wild-type (WT) controls, PiZ and PIZ mice deleted for Chop (PiZ/Chop-/-)

Transgenic PiZ mice have been genetically engineered to express ATZ and have been a valuable experimental model for liver disease due to AAT polymerization. ATZ accumulates in these mice within the endoplamic reticulum (ER) of hepatocytes in a nearly identical manner to livers of affected patients. To investigate the role of the transcription factor CHOP in the pathogenesis of liver damage induced by ATZ, we performed RNA-seq in livers of 6-week-old wild type, PiZ and PiZ mice deleted for Chop. All groups were matched for the strain, age, and the gender. RNA sequencing in 6 week-old wild-type (WT), PiZ and PIZ deleted for Chop (PiZ/Chop-/-) mouse livers mice using QuantSeq 3'mRNA-Seq

转基因PiZ小鼠（Transgenic PiZ mice）经基因工程改造以表达ATZ，是α1-抗胰蛋白酶（AAT）聚合相关性肝病的珍贵实验动物模型。ATZ在该类小鼠肝细胞的内质网（endoplasmic reticulum, ER）中蓄积的模式，与患病患者肝脏的表现几乎完全一致。为探究转录因子CHOP（transcription factor CHOP）在ATZ诱导的肝损伤发病机制中的作用，本研究对6周龄野生型、PiZ以及敲除Chop的PiZ小鼠的肝脏开展了RNA测序（RNA-seq）。所有实验组均在品系、年龄与性别上保持匹配。本研究采用QuantSeq 3' mRNA测序技术，对6周龄野生型（WT）、PiZ以及敲除Chop的PiZ（PiZ/Chop-/-）小鼠肝脏完成了RNA测序。