Direct comparison between a Burkholderia cenocepacia glycosylation mutant strain, and a glycosylation knockdown strain

To determine whether CRISPR interference can be used to recapitulate a glycosylation null mutant strain in Burkholderia cenocepacia via data independent acquisition mass spectrometry本研究旨在通过数据非依赖性采集质谱（data independent acquisition mass spectrometry）技术，探究CRISPR干扰（CRISPR interference）是否可用于在洋葱伯克霍尔德菌（Burkholderia cenocepacia）中复刻糖基化缺失突变菌株。