Transcriptomes of novel human genomic safe harbor sites for gene and cell therapies

Bulk RNA-sequencing experiments were performed to analyze the transcriptomic effects of such integrations into two newly established genomic safe harbor sites. Jurkat and HEK293T cells were edited to integrate CMV-mRuby expressing cassette into Rogi2 genomic safe harbor site using Cas9 RNP

本研究开展批量RNA测序（bulk RNA-sequencing）实验，以分析外源片段整合至两个新建基因组安全港位点（genomic safe harbor sites）所产生的转录组效应；实验中，研究人员采用Cas9核糖核蛋白复合物（Cas9 RNP），将表达CMV-mRuby的表达盒整合至Rogi2基因组安全港位点，以此对Jurkat细胞与HEK293T细胞进行基因编辑。