Drosophila Divergence 20091001

D. yakuba, D. simulans, and D. sechellia gDNA competitively hybridized against D. melanogaster to evaluate aCGH as a means to identify diverged orthologs. 2 D. sechellia vs D. melanogaster hybs - with dye swap, 2 D. simulans vs D. melanogaster hybs with dye swap, and 8 D. yakuba vs D. melanogaster hybs with balanced dye swaps. D. yakuba vs. D. melanogaster were then analyzed in all 2,4,6,8 possible combinations that incorporated dye-swap to asses sources of variation.

以黑腹果蝇（Drosophila melanogaster, D. melanogaster）为杂交参照，对雅库巴果蝇（Drosophila yakuba, D. yakuba）、拟暗果蝇（Drosophila simulans, D. simulans）与塞氏果蝇（Drosophila sechellia, D. sechellia）的基因组DNA（genomic DNA, gDNA）开展竞争性杂交实验，以评估阵列比较基因组杂交（array Comparative Genomic Hybridization, aCGH）用于识别分化型直向同源基因的可行性。本实验共设置3类杂交组合：2组塞氏果蝇vs黑腹果蝇杂交组（均设置染料交换处理）、2组拟暗果蝇vs黑腹果蝇杂交组（均设置染料交换处理），以及8组雅库巴果蝇vs黑腹果蝇杂交组（采用平衡染料交换设计）。随后针对雅库巴果蝇与黑腹果蝇的杂交样本，采用所有包含染料交换的2重、4重、6重、8重可行组合方案进行分析，以评估变异来源。