SPRUCE Methylotrophic Methanogenesis in Sphagnum-dominated Peatland Soils – CH4 and CO2 Production in Laboratory Incubations

This study investigated the potential for methylotrophic methanogenesis in three Sphagnum-dominated peatland soils in northern Minnesota. Soils collected from 2014-06-01 to 2015-06-30 were amended with 13C-labeled traditional substrates (acetate and sodium bicarbonate/ H2) and methylated substrates (methanol, monomethylamine ("MMA"), dimethylsulfide ("DMS")) and monitored for 13C-CH4, 13C-CO2, and net CH4 and CO2 production in laboratory incubations. The peatlands included in the study were (1) the S1 Bog, home to the SPRUCE Experiment and located at the Marcell Experimental Forest (MEF, U.S. Forest Service), (2) Bog Lake Fen, also located at the MEF, and (3) Zim Bog. These sites have been described in detail previously (Medvedeff et al., 2015). This dataset contains one file in comma separate (*.csv) format.

本研究针对明尼苏达州北部三处以泥炭藓（Sphagnum）为主导的泥炭地土壤，探究了甲基营养型产甲烷作用（methylotrophic methanogenesis）的发生潜力。研究于2014年6月1日至2015年6月30日期间采集土壤样品，向样品中添加13C标记的传统底物（乙酸盐与碳酸氢钠/氢气）及甲基化底物（甲醇、一甲胺（MMA）、二甲基硫醚（DMS）），并在实验室培养体系中对13C标记甲烷（13C-CH4）、13C标记二氧化碳（13C-CO2）以及甲烷与二氧化碳的净产生量开展监测。本研究涉及的三处泥炭地分别为：(1) S1泥炭沼泽（S1 Bog），该场地为SPRUCE实验基地，坐落于马塞尔实验林场（Marcell Experimental Forest, MEF，美国林务局）；(2) 博格湖沼泽（Bog Lake Fen），同样位于MEF；(3) 齐姆泥炭沼泽（Zim Bog）。上述采样点的详细信息已在既往研究中详细阐述（Medvedeff等，2015）。本数据集包含1个逗号分隔值（*.csv）格式的文件。