A novel lncRNA GAS1 promotes gastric carcinogenesis and acts as a modular scaffold of WDR5 and KAT2A complexes to specify the histone modification pattern [RNA-seq]. Homo sapiens

To elucidate whether GAS1 plays a role in gastric cancer tumorigenesis, a RNA-seq analysis was performed to compare the gene expression profiles of GAS1 shRNA and control shRNA transfectants. Overall design: RNA-seq was performed in BGC 823 gastric cancer cells after GAS1 shRNA and control shRNA virus infection

为阐明GAS1是否在胃癌肿瘤发生过程中发挥作用，本研究开展RNA测序（RNA-seq）分析，对比了转染靶向GAS1的短发夹RNA（shRNA）与对照短发夹RNA（shRNA）的细胞的基因表达谱。实验整体设计：在BGC 823胃癌细胞中分别感染GAS1 shRNA与对照shRNA病毒后，进行RNA测序分析。