Rodent malaria parasite RNA does not affect mouse BeadChip results

Rodent malaria parasite RNA hybridized on Illumina Mouse WG-6 v2.0 Expression BeadChip To investigate whether parasite RNA interfere with mouse beadchip analaysis. Malaria parasite resides in red blood cell, therefore RNA isolated from whole infected blood contains host RNA as well as parasite RNA Parasite RNA was isolated after leukocyte depletion and RBC lysis, hyridisated to mouse beadchip array as other naïve or infected blood samples

本数据集包含在Illumina小鼠全基因组表达芯片v2.0（Illumina Mouse WG-6 v2.0 Expression BeadChip）上进行杂交的啮齿类疟原虫RNA样本。本实验旨在探究疟原虫RNA是否会对小鼠基因芯片分析造成干扰。疟原虫寄生于红细胞内，因此从受感染全血中分离得到的RNA同时包含宿主RNA与疟原虫RNA。研究人员先通过白细胞去除与红细胞裂解步骤分离得到疟原虫RNA，随后将其与其他未感染及感染血液样本一同进行小鼠基因芯片杂交。