Response of Rhodopirellula baltica to cold shock. Rhodopirellula baltica

Expression profiling of the stress response of the marine Planctomycete Rhodopirellula baltica after a temperature up-shift. R. baltica cultures were grown at 28°C and then shifted to 6°C. Samples were taken after 10, 20, 40, 60 and 300min and compared with cultures before the up-shift (28°C). Overall design: Reference design, time series experiment Whole Genome Array 5 time points, 3 replicates per time point. 3 self-self reference hybridizations. In total, 18 samples.

本数据集针对海洋浮霉菌（Planctomycete）Rhodopirellula baltica在温度升高处理后的应激反应开展表达谱分析。该菌株的培养物先在28℃条件下培养，随后转移至6℃环境中。分别在温度升高处理后的10、20、40、60及300分钟时采集样本，并与处理前（28℃）的培养物进行对照比较。实验整体设计：采用参考设计的时间序列实验方案，使用全基因组芯片（Whole Genome Array）进行检测；共设置5个时间点，每个时间点设置3次生物学重复，同时开展3次自身-自身对照杂交（self-self reference hybridizations）实验，总计18个样本。