H9 hES cells vs. universal RNAs. Homo sapiens

Comparison of human embryonic stem cell transcriptome with universal RNA pool (10 human cell lines) to reveal hES cell-specific gene expression. Keywords: cell type comparison Overall design: RNA was isolated from three replicate exponentially growing cultures of undifferentiated human embryonic stem cells (feeder-free, in MEF-conditioned medium). Human universal RNA was purchased from Stratagene.

为揭示人类胚胎干细胞（human embryonic stem cell, hES）特异性基因表达特征，将人类胚胎干细胞转录组与通用RNA池（含10种人类细胞系）开展对比分析。关键词：细胞类型比较。实验设计概况：从3份生物学重复的、采用无饲养层培养体系、以小鼠胚胎成纤维细胞（mouse embryonic fibroblast, MEF）条件培养基培养的未分化人类胚胎干细胞的指数生长期培养物中分离RNA。通用人类RNA购自Stratagene公司。