Gene expression changes induced by HA-PRCC-TFE3 in HK-2 cells

PRCC-TFE3 is an oncogenic chimeric transcription factor identified in human TFE3-rearranged renal cell carcinoma. To analyze gene expression changes driven by PRCC-TFE3, we generated and utilized an HA-tagged PRCC-TFE3-inducible HK-2 cell line, which expresses HA-tagged PRCC-TFE3 in a doxycycline-dependent manner. Cells were cultured with or without doxycycline, and comprehensive gene expression analysis was conducted using RNA-seq. Overall design: We performed RNA-seq analysis on HA-PRCC-TFE3 doxycycline-inducible HK-2 cell lines, cultured with or without doxycycline.

PRCC-TFE3是一种致癌性嵌合转录因子，在人类TFE3重排肾细胞癌中被鉴定得到。为解析PRCC-TFE3驱动的基因表达变化，我们构建并应用了HA标签的PRCC-TFE3诱导型HK-2细胞系，该细胞系可通过多西环素（doxycycline）依赖的方式表达HA标记的PRCC-TFE3。将该细胞系分别在含多西环素与不含多西环素的培养基中培养，并通过RNA-seq开展全面的基因表达分析。实验整体设计：我们对分别在含或不含多西环素的培养基中培养的HA-PRCC-TFE3诱导型HK-2细胞系进行了RNA-seq分析。