The conserved 3' UTR-derived small RNA NarS mediates mRNA crossregulation during nitrate respiration

In pursuit of a biological role of Salmonella 3' UTR derived sRNA NarS ,we sought to determine potential target mRNAs of NarS under anaerobic conditions. To this end, we compared gene expression in NarS-deficient and NarS overexpression (from plasmid pPL-NarS) strains following a 30-minute anaerobic shock by RNA-seq.

为探究沙门氏菌3'非翻译区（3' UTR）来源的小RNA（small RNA, sRNA）NarS的生物学功能，我们旨在明确厌氧条件下NarS的潜在靶标mRNA。为此，我们通过RNA测序（RNA-seq）对比了经30分钟厌氧冲击处理后的NarS缺陷菌株与由质粒pPL-NarS介导NarS过表达的菌株的基因表达水平。