Rhodococcus erythropolis and Pseudomonas aeruginosa coculture under Al exposure

Raw data from three parallel transcriptomes of Rhodococcus erythropolis and Pseudomonas aeruginosa coculture in 0.1 mM Al3+. The quality of the extracted RNA was assessed using a Nanodrop 2000 and an Agilent 4200 Tape Station bioanalyzer. Only RNA samples with an RNA integrity value (RIN) ≥7 were selected for cDNA library construction. The cDNA library fragments were purified using the AMPure XP system to ensure a preferred length of 400-500 bp. The number of PCR cycles was adjusted to 15, and the final amplified library was quality checked using a Bioanalyzer 2100 system. Finally, an equimolar library was constructed using the Kapa-sybr FAST qPCR Kit Light Cycler 480 and a reference standard from Kapa Biosystems.
Each library was sequenced in paired-end mode using the TruSeq SBS kit v3-HS with a read length of 2 × 76 bp on the HiSeq2000 instrument (Illumina) according to the manufacturer's protocol for mRNA sequencing experiments. The R. erythropolis and P. aeruginosa genomes (GenBank assembly accession numbers: GCA_001715845.1 and GCA_016743035.1) were used as the reference genome.

本数据集包含0.1 mM铝离子（Al³+）胁迫下红平红球菌（Rhodococcus erythropolis）与铜绿假单胞菌（Pseudomonas aeruginosa）共培养的3个平行转录组原始数据。提取的RNA质量通过Nanodrop 2000超微量紫外分光光度计与安捷伦4200 TapeStation生物分析仪进行质检。仅选取RNA完整性值（RIN）≥7的RNA样品开展cDNA文库构建。使用AMPure XP磁珠纯化系统对cDNA文库片段进行纯化，以获得400~500 bp的目标片段长度。将PCR循环数调整为15次，最终扩增得到的文库通过Bioanalyzer 2100系统完成质量验证。最后，采用Kapa-sybr FAST qPCR试剂盒、Light Cycler 480荧光定量PCR仪及Kapa Biosystems提供的参考标准品构建等摩尔混合文库。
每个文库均按照Illumina官方的mRNA测序实验流程，在HiSeq2000测序仪（Illumina）上采用TruSeq SBS v3-HS测序试剂盒进行双端测序，测序读长为2×76 bp。本研究以红平红球菌（Rhodococcus erythropolis）和铜绿假单胞菌（Pseudomonas aeruginosa）的基因组（GenBank组装登录号分别为GCA_001715845.1与GCA_016743035.1）作为参考基因组。