Raw sequencing reads in VDJ project

Noncoding RNA surveillance pathway during B cell development uniquely relates RNA processing with antigen receptor locus programmed DNA rearrangements. For this purpose, we generated mouse models harboring a B cell specific Cre allele Mb1cre, coupled to conditional inversion-deletion (COIN) alleles of exosome core component (Exosc3) and RNase catalytic subunits (Exosc10 and Dis3). We sequenced the transcriptome (RNA-seq) and transposase-accessible chromatin (ATAC-seq) from Dis3COIN/+ and Dis3COIN/COIN B cells.

B细胞发育过程中的非编码RNA监视通路，可将RNA加工过程与抗原受体基因座的程序性DNA重排建立独特关联。为此，我们构建了携带有B细胞特异性Cre重组酶等位基因Mb1cre的小鼠模型，并结合了外泌体核心组分（Exosc3）以及RNase催化亚基（Exosc10与Dis3）的条件性翻转删除（COIN）等位基因。我们对Dis3COIN/+与Dis3COIN/COIN基因型B细胞的转录组（RNA-seq）以及转座酶可及性染色质（ATAC-seq）进行了测序。