Progesterone, via yes-associated protein (YAP), promotes cardiomyocyte proliferation and cardiac repair

To explore effect of progesterone on gene expression of primary cultured cardiomyocytes isolated from 7-day-old SD rats Overall design: We isolated and cultured cardiomyocytes using 7-day-old SD rats and treated cells with control vehicle (control) or progesterone (10-7 M) (progesterone) for 24 hours to prepare sample (three biological replicates for each group) and RNA-sequencing (RNA-Seq) were performed using mRNA extracted from these samples.

为探讨孕酮对7日龄SD大鼠原代培养心肌细胞的基因表达影响。整体实验设计：我们从7日龄SD大鼠中分离并培养心肌细胞，将细胞分为两组，分别以对照溶剂（对照组）或孕酮（10⁻⁷ M，孕酮组）处理24小时以制备实验样本，每组设置3个生物学重复；随后提取各组样本中的mRNA，进行RNA测序（RNA-sequencing，RNA-Seq）。