Profiling sex-biased and developmental gene expression in Daphnia pulex

We use a custom microarray for the crustacean Daphnia pulex to investigate gene expression in males, juvenile females and pregnant females. Keywords: sex-biased, developmental Eight biological replicates of 8 day old healthy male Daphnia pulex are compared to four biological replicates of 8 day old healthy (pregnant) females, or to four biological replicates of healthy 6 day old (juvenile) females. Each comparison involved two dye swaps: two males (green) vs. two pregnant females (red), two males (red) vs. two pregnant females (green) and so on.

本研究针对甲壳类动物蚤状溞（Daphnia pulex）采用定制化基因芯片（custom microarray），旨在探究雄性、幼龄雌性与妊娠雌性的基因表达模式。关键词：性别偏倚表达、发育相关。本研究设置两组独立比对：将8日龄健康雄性蚤状溞的8组生物学重复（biological replicates），分别与4组8日龄健康妊娠雌性的生物学重复、4组6日龄健康幼龄雌性的生物学重复进行比对。每组比对均采用两轮染料互换（dye swap）实验设计：具体为2份雄性样本（标记绿色荧光）与2份妊娠雌性样本（标记红色荧光）、2份雄性样本（标记红色荧光）与2份妊娠雌性样本（标记绿色荧光），以此类推。