Raw Western blot data supporting: "Neutrophil-derived EVs drive NF-κB-dependent endothelial activation and tumor angiogenesis"

This dataset contains raw Western blot images supporting the results presented in Figures 2, 3, and 6 of the associated manuscript. The data include protein expression analyses performed in ER-Hoxb8-derived neutrophils (WT and Ifnar1−/−) as well as neutrophil-derived small extracellular vesicles (NEX), collected at different stages of differentiation (D4-D6). The dataset comprises: total protein gels used as loading controls, Western blot analyses of IFNAR1, exosomal markers (CD63, CD81, CD9, TSG101), intracellular and organelle-associated proteins (PHB1, GM130, Grp94), neutrophil marker (Ly-6G), and signaling pathway components related to NF-κB activation (phospho-p65 (S536), total p65, phospho-IκBα, total IκBα) analyzed in cytosolic and nuclear fractions. For signaling experiments, samples include control conditions and neutrophil/NEX-treated groups analyzed at different time points (6 h and 24 h). Each lane corresponds to individual experimental conditions as indicated in the associated manuscript figures (e.g., WT vs Ifnar1−/−, differentiation stages, treatment conditions). Molecular weight markers (kDa) are indicated where applicable. Detailed experimental procedures, sample preparation, and data analysis are described in the associated publication.本数据集包含支撑关联论文中图2、图3及图6所呈现结果的原始蛋白质免疫印迹（Western blot）图像。 本数据集所涵盖的蛋白质表达分析，取材于ER-Hoxb8来源的中性粒细胞（野生型（Wild Type, WT）及Ifnar1基因敲除型Ifnar1−/−）以及中性粒细胞来源的小型细胞外囊泡（NEX），样本采集于细胞分化的不同阶段（D4至D6）。 本数据集包含以下内容： - 用作上样对照的总蛋白凝胶； - 针对IFNAR1的蛋白质免疫印迹分析； - 外泌体标志物（CD63、CD81、CD9、TSG101）； - 细胞内及细胞器相关蛋白（PHB1、GM130、Grp94）； - 中性粒细胞标志物Ly-6G； - 针对胞质与核组分中核因子κB（NF-κB）活化相关信号通路分子的分析，所涉分子包括磷酸化p65（S536位点）、总p65、磷酸化IκBα及总IκBα。 在信号通路相关实验中，样本涵盖对照组及经中性粒细胞/NEX处理的组别，并于6小时、24小时两个不同时间点进行检测分析。 每一条泳道对应关联论文配图中所示的单一实验条件（例如野生型WT与Ifnar1−/−的对比、细胞分化阶段、处理条件等）。分子质量标志物（单位为千道尔顿，kDa）在适用场景下已予以标注。 详细的实验流程、样本制备方法及数据分析过程已在关联发表论文中予以详述。