HSP27 3A vs WT. Homo sapiens

human bronchial smooth muscle cells were infected with adenoviral constructs containing either the phosphorylation mutant form of HSP27 (HSP27-3A) or the wild type form (HSP27-WT) at 40 MOI for 4 days. All sets were stimulated with a cytokine mixture containing 10 ng/ml IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to harvest. This series represents 4 separate preparations of cells and RNA, with each set being subjected to a dye flip and hybridized to 2 different slides. Keywords: parallel sample

将人类支气管平滑肌细胞以感染复数（Multiplicity of Infection, MOI）为40的剂量，分别用携带HSP27磷酸化突变体（HSP27-3A）或野生型HSP27（HSP27-WT）的腺病毒载体感染，孵育4天。
所有实验组均在样本收获前20小时，采用含有10 ng/ml白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）及γ干扰素（IFN-γ）的细胞因子混合物进行刺激。
本数据集共包含4组独立的细胞与RNA制备样本，每组样本均进行染料翻转处理，并与两张不同的玻片完成杂交。
关键词：平行样本（parallel sample）