Genome-wide maps of CtBP binding profile in breast cancer cell line MCF-7. Homo sapiens

CtBP is a global co-repressor by serving as transcriptional factor in multiple pathways. CtBP functioned as transcriptional factor by recruiting other cofactors such as G9a, HDAC1 and PcG proteins. CtBP is found to be over enriched in several type of tumor samples. To dipict the role of CtBP in globally regulating gene expression, we applied ChIP-seq technology to find out the binding profile of CtBP in breast cancer cell line MCF-7. Our data suggest CtBP plays a global regulatory role in DNA damage repair, tumor initiation, and EMT process. Overall design: Examine CtBP binding in MCF-7 cells

CtBP是一种全局性共抑制因子，可通过在多条信号通路中充当转录因子发挥功能。CtBP可通过招募G9a、HDAC1及PcG蛋白等其他辅因子行使转录因子的功能。研究发现，CtBP在多种肿瘤样本中呈现显著富集。为阐明CtBP在全局调控基因表达中的作用，我们采用染色质免疫共沉淀测序（ChIP-seq）技术，解析了CtBP在乳腺癌细胞系MCF-7中的结合谱。我们的数据表明，CtBP在DNA损伤修复、肿瘤起始以及上皮间质转化（EMT）过程中发挥全局性调控作用。整体实验设计：检测MCF-7细胞中CtBP的结合情况。