Immune Gene Expression Profile of Tr1 Skewed Tregs. Immune Gene Expression Profile of Tr1 Skewed Tregs

IL-12 and IL-18 synergize to promote TH1 responses and have been implicated as accelerators of autoimmune pathogenesis in type 1 diabetes (T1D). We therefore investigated the influence of these cytokines on phenotype and function of immune cells that are involved in disease progression. To understand how IL-12 and IL-18 may synergize to impair Treg function and phenotype, we conducted transcriptional profiling of Treg expanded under normal conditions or in the presence of IL-12 and IL-18. This analysis revealed increased expression of IFNG, GZMB, GZMA, and IL18RAP and decreased FOXP3 in Tregs expanded with IL-12 and IL-18. Overall design: Transcriptional profiling of Treg expanded under normal conditions or in the presence of IL-12 and IL-18. 3 samples, 2 conditions each

白细胞介素12（Interleukin-12, IL-12）与白细胞介素18（Interleukin-18, IL-18）可协同诱导辅助性T细胞1（T helper 1, TH1）应答，且被证实为1型糖尿病（Type 1 Diabetes, T1D）自身免疫发病进程的加速因子。本研究就此探究了上述细胞因子对参与疾病进展的免疫细胞表型与功能的影响。为阐明IL-12与IL-18如何协同削弱调节性T细胞（Regulatory T cell, Treg）的功能与表型，我们对在常规培养条件下，或添加IL-12与IL-18的培养液中扩增的Treg开展了转录谱分析。该分析结果显示，经IL-12与IL-18扩增的Treg中，IFNG、GZMB、GZMA及IL18RAP的表达水平显著上调，而FOXP3的表达水平显著下调。实验整体设计：对在常规培养条件下，或添加IL-12与IL-18的培养液中扩增的Treg进行转录谱分析，共设置2种培养条件，每种条件包含3个生物学重复样本。