Visceral adipose tissue macrophage subsets

We identified two different subsets of macrophages in visceral adipose tissue. These two subsets were transcriptomically different. We observed that C3CR1-low CCR2-low macrophages are transcriptomically different after myocardial infarction (MI). Overall design: We sorted CX3CR1-high CCR2-high and CX3CR1-low and CCR2-low macrophages from visceral adipose tissue and blood monocytes from mice in the steady state, and CX3CR1-low and CCR2-low macrophages from visceral adipose tissue of mice with MI or sham surgery directly in RNA extraction buffer (Applied Biosystems Pico Pure Kit for mRNA extraction) and performed RNA-seq analysis.

我们在内脏脂肪组织（visceral adipose tissue）中鉴定出两种不同的巨噬细胞（macrophages）亚群，二者在转录组层面存在显著差异。研究观察到，在心肌梗死（myocardial infarction, MI）发生后，C3CR1低表达、CCR2低表达的巨噬细胞同样呈现转录组层面的特异性差异。实验设计概述：我们从生理稳态小鼠的内脏脂肪组织中分选得到CX3CR1高表达、CCR2高表达以及CX3CR1低表达、CCR2低表达的巨噬细胞，同时分离获取该类小鼠的血液单核细胞；此外，我们直接从接受心肌梗死造模或假手术的小鼠的内脏脂肪组织中获取CX3CR1低表达、CCR2低表达的巨噬细胞，将其置于RNA提取缓冲液中（使用Applied Biosystems Pico Pure试剂盒完成mRNA提取），随后开展RNA测序（RNA-seq）分析。