MicroCT of stained bovine IVD quarter segments after 2 weeks incubation

Visualising the 3D microstructure of stained and native intervertebral discs using X-ray microtomography C.M. Disney 1,2, K. Madi 3, A.J. Bodey 4, P.D. Lee 3, J.A. Hoyland 2,5, M.J. Sherratt 2 1 Centre for Doctoral Training in Regenerative Medicine, 2 Division of Cell Matrix Biology and Regenerative Medicine, 3 School of Materials, University of Manchester, UK. 4 Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, UK. 5 NIHR Manchester Biomedical Research Centre, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, UK. A comparison of three stains; iodine potassium iodide (I2KI), phosphomolybdic acid (PMA) and phosphotungstic acid (PTA). Chemically fixed (10% formal saline) quarter segments of bovine tail intervertebral disc were incubated for two weeks before being imaged by laboratory microCT. Reconstruction software (Phoenix dato s|x2 reconstruction) was used to generate 3D datasets from the projections. This reconstructed data relates to figure 3 and is saved as 8-bit .raw with image dimensions given in the file name.

利用X射线显微计算机断层扫描（X-ray microtomography）可视化染色与天然椎间盘的三维微观结构

作者：C.M. Disney, K. Madi, A.J. Bodey, P.D. Lee, J.A. Hoyland, M.J. Sherratt
单位：1 英国曼彻斯特大学再生医学博士培训中心；2 细胞基质生物学与再生医学学部；3 英国曼彻斯特大学材料学院；4 英国牛津郡哈维尔科学与创新园区钻石光源（Diamond Light Source）；5 英国曼彻斯特学术健康科学中心、中央曼彻斯特大学医院国民保健服务基金会NIHR曼彻斯特生物医学研究中心

本研究对比了三种染色剂：碘化钾碘溶液（iodine potassium iodide，I2KI）、磷钼酸（phosphomolybdic acid，PMA）以及磷钨酸（phosphotungstic acid，PTA）。将经10%甲醛生理盐水化学固定的牛尾椎间盘四分体标本孵育两周后，采用实验室显微CT进行成像。使用Phoenix Datos|X2重建软件从投影数据中生成三维数据集。本部分重建数据对应图3，以8位.raw格式存储，图像尺寸信息包含在文件名中。