RNA-Seq of 3iL human embryonic stem cells. RNA-Seq of 3iL human embryonic stem cells

Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts. The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine. Even though hESCs are pluripotent, differences have been observed when compared to the native pluripotent epiblast cells of the blastocyst. We use a chemical approach (3iL: 3 small molecule inhibitor and cytokine) to induce an expression signature that more closely resembles native pluripotent cells. To better understand the difference between this hESC state and the conventional hESC state, we generated the global expression profiles using RNA-seq.

人类胚胎干细胞（hESCs）分离自囊胚的内细胞团。其多能性特性可分化获得不同谱系的细胞类型或组织，有望应用于药物研发与再生医学等领域。尽管hESCs具备多能性，但与囊胚的天然多能上胚层细胞相比，已观测到二者存在差异。本研究采用化学方法（3iL：3种小分子抑制剂与细胞因子）诱导出与天然多能细胞特征更为相似的表达特征。为进一步明晰该hESC状态与传统hESC状态之间的差异，我们通过RNA测序（RNA-seq）构建了全局表达谱。