Primers for specific amplification of Seg-2 of BTV-6, by RT-PCR.

*Individual primers are identified by the BTV serotype (e.g. BTV-6) followed by the number 2 (to indicate Seg-2), then a number to indicate the relative amino acid position of the primer within VP2, followed by F or R to indicate forward or reverse orientation.
**This primer pair also amplifies Seg-2 of BTV-14 and BTV-21, the most closely related serotypes to BTV-6 within nucleotype ‘C’ and it is therefore regarded as nucleotype ‘C’ specific.

* 单个引物的命名规则为：以蓝舌病病毒（Bluetongue Virus，BTV）血清型（例如BTV-6）为前缀，紧随其后添加数字2（用于标识第2基因组节段Segment 2，Seg-2），再接入一个数字以指示该引物在VP2（病毒衣壳蛋白2，Viral Protein 2）内的相对氨基酸位置，最后以F或R分别表示正向引物与反向引物。
** 该引物对还可扩增BTV-14与BTV-21的第2基因组节段（Seg-2）；这两种血清型是核型‘C’中与BTV-6亲缘关系最为密切的毒株，因此该引物对被认定为核型‘C’特异性引物。