Additional file 1 of FOXC1 promotes HCC proliferation and metastasis by Upregulating DNMT3B to induce DNA Hypermethylation of CTH promoter

Additional file 1: Supplementary Table S1. List of genes differentially expressed in Huh7-FOXC1 versus Huh7-control cells using a human amino acid metabolism PCR array. Supplementary Table S2. List of genes differentially expressed in MHCC97H-shFOXC1 versus MHCC97H-shcontrol cells using a human amino acid metabolism PCR array. Supplementary Table S3. Correlation between CTH methylation and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S4. Correlation between DNMT3B expression and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S5. Correlation between 8-OHdG expression and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S6. Correlation between pELK1 expression and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S7. Primer sequences used in the study. Supplementary Table S8. Knockdown shRNA sequences used in this study.

附加文件1：
补充表S1：采用人类氨基酸代谢聚合酶链式反应（PCR）芯片筛选得到的Huh7-FOXC1与Huh7-对照细胞间的差异表达基因列表
补充表S2：采用人类氨基酸代谢聚合酶链式反应（PCR）芯片筛选得到的MHCC97H-shFOXC1与MHCC97H-sh对照细胞间的差异表达基因列表
补充表S3：在两个人类肝细胞癌（Hepatocellular carcinoma，HCC）组织独立队列中，胱硫醚γ裂解酶（CTH）基因甲基化与HCC临床病理特征的相关性分析结果
补充表S4：在两个人类HCC组织独立队列中，DNA甲基转移酶3B（DNMT3B）表达与HCC临床病理特征的相关性分析结果
补充表S5：在两个人类HCC组织独立队列中，8-羟基脱氧鸟苷（8-OHdG）表达与HCC临床病理特征的相关性分析结果
补充表S6：在两个人类HCC组织独立队列中，磷酸化ELK1（pELK1）表达与HCC临床病理特征的相关性分析结果
补充表S7：本研究使用的引物序列
补充表S8：本研究使用的敲低短发夹RNA（shRNA）序列