Glycan signal enhancement by ammonium fluoride doping and electrospray diverting

The dataset contains information demonstrating that the inclusion of ammonium fluoride in the electrospray and use of an electrospray diverter with optimized timing helps to significantly increase signal of glycans. This shows up to a 131-fold increase in signal in tissue and a 30-fold increase in solution.  , , # Glycan signal enhancement by ammonium fluoride doping and electrospray diverting Dataset DOI: [10.5061/dryad.kkwh70shx](https://doi.org/10.5061/dryad.kkwh70shx) This dataset contains mass spectrometry imaging datasets derived from the imaging of kidney samples and from prepared solutions from bovine fetuin. For the image analysis, the necessary imzML, ibh, and ibd files are provided. Load this data into image viewing software such as MSiReader. For the solution analysis, the raw data can be uploaded to an image viewing software or the raw files can be viewed directly in Freestyle.  In this work, we utilize doped electrospray with 350 uM ammonium fluoride and an electrospray diverter to investigate their impact on glycan signal. This used 4 tissues and 3 solution samples. The abundance of observed glycans between the two conditions were compared to evaluate a signal enhancement factor.  ## Description of the data and file structure Data was generated by imaging prepared N-glycan..., ,# 氟化铵掺杂与电喷雾分流技术实现聚糖信号增强 该数据集收录的信息表明，在电喷雾体系中掺入氟化铵（ammonium fluoride），并使用时序优化的电喷雾分流器（electrospray diverter），可显著提升聚糖（glycans）的信号强度。实验结果显示，在组织样本中信号最高可提升131倍，在溶液体系中最高可提升30倍。 数据集DOI：[10.5061/dryad.kkwh70shx](https://doi.org/10.5061/dryad.kkwh70shx) 本数据集包含两类质谱成像（mass spectrometry imaging）数据集：一类源自肾脏组织样本的成像分析，另一类源自牛胎球蛋白（bovine fetuin）的预制溶液分析。针对成像分析，已提供所需的imzML、ibh及ibd格式文件，可将此类数据导入MSiReader等成像查看软件中进行分析。针对溶液分析，原始数据可上传至成像查看软件，或直接在Freestyle软件中打开原始文件进行观测。 本研究采用350 μM氟化铵掺杂的电喷雾体系配合电喷雾分流器，探究二者对聚糖信号的调控作用。实验共设置4组组织样本与3组溶液样本，通过对比两种实验条件下观测到的聚糖丰度，计算得到信号增强因子。 ## 数据与文件结构说明 本数据集通过预制N-聚糖（N-glycan）的成像实验生成……