Human pluripotent stem cell-derived astrocyte model in fragile X syndrome. Human pluripotent stem cell-derived astrocyte model in fragile X syndrome

The experiment was made to compare gene expression of human control (H1) ES-derived astrocytes and isogenic astrocytes carrying a mutation causing fragile x syndrome. Overall design: Gene expression profiling was performed using data obtained from RNA-seq of triplicate samples at 5 time points (Day 0 (D0), D12, D30, D60 and D95) during forebrain astrocyte differentation.

本实验旨在比较人类对照（H1）胚胎干细胞（Embryonic Stem, ES）来源星形胶质细胞，与携带致脆性X综合征（Fragile X Syndrome）突变的同基因星形胶质细胞的基因表达差异。实验整体设计：在前脑星形胶质细胞分化过程中，于5个时间点（第0天（D0）、D12、D30、D60及D95）采集三份重复样本，通过RNA测序（RNA-seq）获取数据以进行基因表达谱分析。