Equus caballus Raw sequence reads

This study evaluates the HIVE scRNA-seq method, a pico-well-based technology for processing bronchoalveolar lavage (BAL) cells from horses with equine asthma (EA). The HIVE method employs pico-well technology, providing a gentle workflow specifically designed for fragile cells. The incorporation of cells stabilized within the pico-wells enables easy storage and subsequent batch processing. The method's rapid and straightforward fixation, facilitated by portable cartridges, simplifies field and clinical sampling. HIVE scRNA-seq libraries were prepared from equine bronchoalveolar lavage (BAL) cells obtained from horses undergoing evaluation for equine asthma. Seventeen HIVE (v1) scRNA-seq libraries, generated from 13 BAL samples, were sequenced on the NovaSeq 6000.

本研究评估了HIVE单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）方法，该技术基于皮克孔（pico-well）平台，用于处理罹患马哮喘（equine asthma, EA）马匹的支气管肺泡灌洗（bronchoalveolar lavage, BAL）细胞。HIVE方法采用皮克孔技术，开发了专为脆弱细胞优化的温和实验流程。将细胞稳定封装于皮克孔内的设计，可实现便捷的样本储存与后续批量处理。该方法依托便携耗材盒即可实现快速简便的细胞固定，大幅简化了野外与临床采样工作。本研究从针对马哮喘开展评估的马匹所获取的支气管肺泡灌洗细胞中，制备了HIVE scRNA-seq文库。共计17个HIVE（v1版本）scRNA-seq文库（源自13个BAL样本）在NovaSeq 6000测序平台上完成测序。