Additional file 2 of Spatial and single-cell expression analyses reveal complex expression domains in early wheat spike development

Additional file 2: Table S1. Gene symbols and identification numbers. Table S2. smFISH cell cluster names and preferential expression. Table S3. Correlations among spatial expression clusters. Table S4. Characterization of stem length and diameter in lfy mutants. Table S5. Differences in hybridization density in the terminal meristems. Table S6. Phenotypic analysis of spl14 CRISPR-induced mutants. Table S7. Description of the scRNA-seq libraries. Table S8. Correlation matrix among single-cell clusters. Table S9. Genes preferentially expressed in the different single-cell clusters. Table S10. KEGG enriched pathways in each cluster. Table S11. Differential expression among basal, central and tip spike regions. Table S12. Projection of smFISH cluster IDs into scRNA-seq cells. Table S13. Correlations scRNA-seq & smFISH-hexaploid at double ridge. Table S14. Correlations scRNA-seq & smFISH-hexaploid at lemma primordia. Table S15. Correlations scRNA-seq & smFISH-hexaploid at terminal spikelet. Table S16. Annotation of single cell clusters. Table S17. Genes preferentially expressed in single-cell epidermal clusters. Table S18. Comparison of subclusters sc18_1and sc18_2. Table S19. Distribution of genes potentially effected by protoplasting. Table S20. Genes co-expressed with FZP-A. Table S21. Genes co-expressed with TCP-A24. Table S22. Network analysis of genes co-expressed with FZP and TCP24. Table S23. Genes co-expressed with ULTRAPETALA-A1. Table S24. Genes co-expressed with AGL-A6. Table S25. Guide RNA sequences and primers for SPL14 and FZP validation.

附加文件2：
表S1 基因符号与识别编号
表S2 单分子荧光原位杂交（single-molecule fluorescence in situ hybridization，smFISH）细胞簇名称及偏好表达基因
表S3 空间表达簇间的相关性
表S4 lfy突变体的茎长与直径特征分析
表S5 顶端分生组织的杂交信号密度差异
表S6 spl14 CRISPR（成簇规律间隔短回文重复序列，Clustered Regularly Interspaced Short Palindromic Repeats）诱导突变体的表型分析
表S7 单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）文库概况
表S8 单细胞簇间的相关矩阵
表S9 不同单细胞簇中的偏好表达基因
表S10 京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集通路
表S11 穗部基部、中部与尖端区域的基因差异表达情况
表S12 smFISH细胞簇ID向scRNA-seq细胞的映射
表S13 双棱期六倍体smFISH与scRNA-seq数据间的相关性
表S14 浆片原基期六倍体smFISH与scRNA-seq数据间的相关性
表S15 顶端小穗期六倍体smFISH与scRNA-seq数据间的相关性
表S16 单细胞簇的注释信息
表S17 单细胞表皮簇中的偏好表达基因
表S18 亚簇sc18_1与sc18_2的比较分析
表S19 原生质体分离潜在影响基因的分布情况
表S20 与FZP-A共表达的基因
表S21 与TCP-A24共表达的基因
表S22 FZP与TCP24共表达基因的网络分析
表S23 与ULTRAPETALA-A1共表达的基因
表S24 与AGL-A6共表达的基因
表S25 用于SPL14与FZP验证的向导RNA序列及引物