Quantitative and FACS analysis of bacterial and fungal communities on building materials

This is a comprehensive study of microbial community on building materials. Using epifluorescence and differential contrast microscopy, we estimated viral, bacterial and fungal abundances on two building materials under high moisture condition. We also studied microbial growth and metabolism on building materials by using flow cytometry-based methods to differentiate between viable and non-viable cells. FACS was performed to collect viable and non-viable cells fractions separately for further sequencing. With the sequence data, we performed quantitative analysis by incorporating sample biomass. We also took a closer look at the bacterial and fungal community together to study their interactions and dynamics.

本研究针对建筑材料表面的微生物群落开展系统性探究。研究借助落射荧光显微镜（epifluorescence microscopy）与微分对比显微镜（differential contrast microscopy），对高湿度环境下两种建筑材料表面的病毒、细菌及真菌丰度进行了定量评估。同时，本研究采用流式细胞术（flow cytometry）区分活菌与死菌，以此解析建筑材料表面微生物的生长与代谢特征。此外，本研究通过荧光激活细胞分选术（FACS）分别分离活菌与死菌组分，用于后续测序实验。基于测序得到的序列数据，本研究结合样本生物量开展定量分析，并针对细菌与真菌群落展开联合解析，以探究二者的相互作用机制及群落动态变化规律。