Comparison of transcriptional profiles of (1) intestinal ILC3s isolated from WT and IL-17D-deficient mice; (2) CD93+ILC3s and CD93-ILC3s from WT mice

IL-17D/CD93 axis was essential for ILC3s hemostasis. In order to further understand how IL-17D-CD93 axis regulate ILC3s function, we conducted RNA-seq analysis of ILC3s. Cell suspension were isolated from intestine of WT or IL-17D-deficient mice under steady state and ILC3s (live CD45lowCD3negCD19negCD90.2high) were sort purified. CD93+ or CD93- ILC3s were sorted from WT mice. RNA was isolated and RNA-seq was performed using BGISEQ-500 platform by BGI Genomics and successfully mapped to mouse genome.

IL-17D/CD93信号轴对3型固有淋巴细胞（ILC3s）的稳态维持至关重要。为进一步解析IL-17D-CD93信号轴调控ILC3s功能的具体机制，我们对ILC3s开展了RNA-seq测序分析。实验于稳态条件下，从野生型（WT）或IL-17D缺陷型小鼠的肠道中分离细胞悬液，并分选纯化得到ILC3s（活细胞表型：CD45lowCD3negCD19negCD90.2high）。此外，我们从野生型小鼠体内分选出CD93阳性（CD93+）与CD93阴性（CD93-）的ILC3s。提取总RNA后，由BGI Genomics采用BGISEQ-500测序平台完成RNA-seq测序，测序数据可成功比对至小鼠基因组。