Analysis of the developmental function of the PAIRED-Like homeobox transcription factor Drgx in T4/T5 neurons of Drosophila melanogaster [RNA-seq]

Targeted DamID was performed to identify binding targets of Drgx in T4/T5 neurons. RNA-seq analysis of differentially expressed genes following the knockdown of Drgx in T4/T5 neurons of late third instar larvae, compared with control. RNAseq of FACS sorted T4/T5 neurons from wandering third instar larvae (control: R23G12 x cherry-IR, experiment: R23G12 x DRGX-IR)

本研究开展靶向DamID（Targeted DamID）实验，以鉴定Drgx在T4/T5神经元中的结合靶点。以晚期三龄幼虫T4/T5神经元中敲低Drgx后的样本与对照组进行对比，对其差异表达基因开展RNA测序（RNA-seq）分析；同时对从漫游期三龄幼虫中经荧光激活细胞分选（FACS）分离得到的T4/T5神经元进行RNA测序，其中对照组为R23G12 × cherry-IR，实验组为R23G12 × DRGX-IR。