Transcriptome analysis of ROP10 GTPase-mediated abscisic acid (ABA) signaling in Arabidopsis. Arabidopsis thaliana

In this study, we have identified genes that are respectively activated and repressed by the low concentration of ABA and show that ROP10 gates a specific subset of genes that are responsive only to a low ABA concentration. Keywords: Whole seedlings of wild-type (Ws) and the mutant (rop10-1) treated with or without ABA Overall design: 12 Arabidopsis ATH1 chips were purchased from Affymetrix. Total RNA was extracted and purified from 1µM ABA-treated or non-treated Ws and rop10-1. Each genotype/treatment has three biological replicates. Probe labeling, array hybridization and scanning were done by the University of California-Irvine DNA Microarray Facility.

本研究鉴定了分别被低浓度脱落酸（Abscisic acid，ABA）激活与抑制的基因，并证实ROP10可调控仅对低浓度ABA产生响应的特定基因子集。
关键词：经ABA处理或未处理的野生型（Ws）及突变体（rop10-1）完整幼苗
实验设计概述：本研究使用购自Affymetrix的12张拟南芥（Arabidopsis）ATH1基因芯片。从经1μM ABA处理或未处理的Ws与rop10-1中提取并纯化总RNA。每个基因型/处理组均设置3个生物学重复。探针标记、芯片杂交及扫描工作由加州大学欧文分校DNA微阵列服务中心完成。