Differential gene expression from azithromycin and SMM treated human airway epithelium. Homo sapiens

Microarrays were used to evaluate the effects of azithromycin and an inflammatory stimulus (SMM) on human airway epithelium. Effects of azithromycin treatment were evaluated at 6, 24 and 48 hours. Effects of SMM were evaluated at 6 and 24 hours. In addition, pretreatment with azithromycin was used to evaluate the modulatory effects on SMM-induced inflammation. SMM=supernatant from microcorpulent material from human cystic fibrosis airways. Keywords: timecourse, treatment comparisons. Overall design: 10 treatments total, 3-4 samples (patient codes = replicates) per treatment.

本研究采用基因芯片（Microarrays）技术，评估阿奇霉素与炎症刺激物(SMM)对人气道上皮细胞的作用效果。具体而言，分别于6、24及48小时三个时间点，检测阿奇霉素处理的细胞效应；分别于6及24小时两个时间点，检测SMM处理的细胞效应。此外，本研究通过阿奇霉素预处理方案，评估其对SMM诱导的炎症反应的调控作用。SMM即人类囊性纤维化气道来源的微稠物质上清液。关键词：时间进程、处理组比较。整体实验设计：共设置10种处理条件，每组包含3-4个生物学重复样本，重复样本以患者编号作为标识。