Identification and Bioinformatic Analysis of CircRNAs in the Plasma of Patients with Very Severe Chronic Obstructive Pulmonary Disease. Identification and Bioinformatic Analysis of CircRNAs in the Plasma of Patients with Very Severe Chronic Obstructive Pulmonary Disease

The differential expression of circular RNAs (circRNAs) in individuals with very severe chronic obstructive pulmonary disease (COPD) and healthy individuals was screened using microarray technology. The related functions and mechanisms were analyzed using bioinformatic methods to explore the potential of target circRNAs as biomarkers of COPD and provide insights for future pathogenesis. Overall design: Blood samples from three patients with very severe COPD and three healthy controls were randomly selected.

本研究采用微阵列（microarray）技术，筛选极重度慢性阻塞性肺疾病（COPD）患者与健康个体间环状RNA（circRNAs）的差异表达情况；通过生物信息学方法分析其相关功能与作用机制，以期探究目标环状RNA作为COPD生物标志物的潜力，并为后续该病发病机制的研究提供参考依据。整体实验设计：随机选取3例极重度慢性阻塞性肺疾病患者与3例健康对照者的血液样本。