Different Diets Change the Bovine Milk Extracellular Vesicle-miRNA Expression

Given that different diets could alter cow milk yield and composition, the effects of different feed formula on milk extracellular vesicle (EV) miRNAs were detected. Cow milk EVs contained various small RNAs, including miRNAs, snRNAs, tiRNAs, Cis-regulatory elements, and piRNAs. Two hundred and seventy-six known bos taurus miRNAs were identified by sequencing in bovine milk EVs. There were 13 immune-related miRNAs in the top 20 miRNAs in milk EVs. Nine differently expressed known miRNAs were detected in responding to different feed formulations. Cow milk EVs are abundant of small RNAs, especially miRNAs, which might be closely related to the development of maternal mammary gland and neonatal immune maturity. Exploration the EV-miRNA profiling changes of bovine milk responding to two different diets

鉴于不同日粮可改变奶牛乳产量与乳成分，本研究检测了不同饲料配方对牛乳细胞外囊泡（extracellular vesicle, EV）microRNA（miRNA）的影响。牛乳EV包含多种小RNA，包括miRNA、小核RNA（snRNA）、转运干扰RNA（tiRNA）、顺式调控元件以及piwi相互作用RNA（piRNA）。通过测序技术在牛乳EV中鉴定出276个已知的牛（Bos taurus）miRNA。在牛乳EV中丰度排名前20的miRNA中，有13个与免疫相关。在不同饲料配方处理下，共检测到9个差异表达的已知miRNA。牛乳EV富含各类小RNA，尤其是miRNA，其可能与母体乳腺发育以及新生动物免疫成熟过程密切相关。本研究探索了牛乳EV-miRNA表达谱在两种不同日粮处理下的变化特征。