HPV cell lines and HPV tissue MicroRNA SOLiD sequencing

Human papillomaviruses (HPV) preferentially infect keratinocytes of mucous membranes or skin and cause numerous benign and malignant lesions at different anatomical locations. We sequenced small RNA (sRNA) libraries of two HPV 16 immortalized cell lines and ten formalin fixed paraffin embedded (FFPE) tissue samples from HPV infected cervical epithelium by SOLiD 4 technology. Overall design: Small RNA (sRNA) libraries of two HPV 16 immortalized cell lines, HPK IA and HPK II, and ten formalin fixed paraffin embedded (FFPE) tissue samples from HPV infected cervical epithelium, were made according to SOLiD sequencing instructions.

人类乳头瘤病毒（Human papillomaviruses, HPV）偏好感染黏膜或皮肤的角质形成细胞，并可在不同解剖部位引发多种良恶性病变。本研究采用SOLiD 4测序技术，对两株HPV16永生化细胞系以及10例HPV感染的宫颈上皮的福尔马林固定石蜡包埋（formalin fixed paraffin embedded, FFPE）组织样本的小RNA（small RNA, sRNA）文库进行了测序。实验整体设计：依照SOLiD测序操作指南，构建了两株HPV16永生化细胞系HPK IA、HPK II，以及10例HPV感染的宫颈上皮的福尔马林固定石蜡包埋（FFPE）组织样本的小RNA（sRNA）文库。