scRNA-seq of tumor-specific CD8+ T cells

Tumor-specific CD8+ T cells are heterogeneous; Tcf1+ stem-like CD8+ T cells give rise to cytotoxic Tim-3+ terminally differentiated CD8+ T cells. The goal of this study is to determine the capacity of CD69 to regulate differentiation of tumor-specific CD8+ T cells in the tumor microenvironment and tumor-draining lymph nodes. Our single-cell RNA-seq and RNA-seq analyses of tumor-specific CD8+ T cells reveal the important function of CD69 in anti-tumor CD8+ T cell immunity. Thus, CD69 is an attractive target for cancer immunotherapy. Overall design: AH1 tetramer+ CD8+ T cells were sorted and subjected to scRNA-seq.

肿瘤特异性CD8+ T细胞（tumor-specific CD8+ T cells）具有异质性；表达Tcf1的干细胞样CD8+ T细胞（Tcf1+ stem-like CD8+ T cells）可分化为细胞毒性Tim-3+终末分化CD8+ T细胞。本研究旨在明确CD69在肿瘤微环境（tumor microenvironment）与肿瘤引流淋巴结（tumor-draining lymph nodes）中调控肿瘤特异性CD8+ T细胞分化的能力。我们针对肿瘤特异性CD8+ T细胞开展的单细胞RNA测序（single-cell RNA-seq）与RNA测序（RNA-seq）分析，揭示了CD69在抗肿瘤CD8+ T细胞免疫中的关键功能。因此，CD69是癌症免疫治疗的极具潜力的靶点。整体实验设计：分选得到AH1四聚体阳性CD8+ T细胞，并对其进行scRNA-seq分析。