Drug-susceptibility and resistance indices (RI) of the drug-susceptible LEM3049 isolate for the antileishmanial reference drugs in the different macrophage cell types.

The 50% inhibitory concentration (IC50) of LEM3049 and the RI against pentavalent and trivalent antimonials (SbV and SbIII), miltefosine (MIL), paromomycin (PMM) and amphotericin B (AmB) were determined in primary peritoneal Swiss mouse macrophages (PECs), BALB/c bone-marrow derived macrophages (BMMφ), human blood mononuclear cell-(PBMC) derived macrophages and the commercially available THP-1 and J774 cell lines (not done for RAW cells). Values are expressed as the average IC50 ± SEM and are the result of two independent tests run in duplicate.

本研究测定了LEM3049的半数抑制浓度（50% inhibitory concentration, IC50），以及其针对五价锑剂（pentavalent antimonials, SbV）、三价锑剂（trivalent antimonials, SbIII）、米替福新（miltefosine, MIL）、巴龙霉素（paromomycin, PMM）与两性霉素B（amphotericin B, AmB）的耐药指数（Resistance Index, RI）。检测对象涵盖瑞士小鼠原代腹腔巨噬细胞（peritoneal exudate cells, PECs）、BALB/c小鼠骨髓来源巨噬细胞（bone-marrow derived macrophages, BMMφ）、人外周血单个核细胞（peripheral blood mononuclear cell, PBMC）来源巨噬细胞，以及商用THP-1和J774细胞系（RAW细胞未开展相关检测）。所有数值以平均IC50 ± 标准误（standard error of the mean, SEM）表示，均为两次独立实验且每次实验设置双复孔的结果。